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KMID : 0359319870270020253
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Korean Journal of Veterinary Research
1987 Volume.27 No. 2 p.253 ~ p.258
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Studies on the Activity of Immune Regulatory Cells in the Korean Native Cattle
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이주묵/Lee, Ju mook
김종면/송희종/채효석/유남선/강명대/Kim, Jong Myeon/Song, Hee Jong/Chai, Hyo Seok/You, Nam Sun/Kang, Myeong Dai
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Abstract
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The erythrocytes (E) rosette forming capacity of peripheral blood lymphocytes(PBL) in Korean native cattle was determined with 2-aminethylisothiouronium bromide hydrobromide (AET) and dextran (Dex) -treated sheep erythrocytes (SRBC). To further standardize the assay, optimum concentration of AET and /or Dex-treatment and incubation time for rosette forming cell (RFC) counts were determined.
In untreated SRBC resuspended in the rosetting medium(RPMI 1640 containing 10% FCS), PBL from 7 animals formed low percentage of rosettes(7.8±6.0%). Both AET and Dex treatment not only enhanced the rosette formation but also made it easy to enumerate rosettes by increasing numbers of SRBC attached on them. SRBC treated with 0.1M AET for 20 min or 8% Dex formed the highest percentages of rosettes, (35.7±6.0% and 48.3±4. 7%, respectively) and were used in subsequent studies.
With SRBC treated with 0.1M AET for 20 min and suspended in 8% Dex, the maximum RFC observed were 57.6±6.8% in female and 75.8±6.3% in male and the rates of RFC did not change significantly between 3 and 20 hr incubation time at 4℃.
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KEYWORD
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